Kinetic plots in aqueous size exclusion chromatography of monoclonal antibodies and virus particles
نویسندگان
چکیده
منابع مشابه
Size-exclusion chromatography using core-shell particles.
Size-exclusion chromatography (SEC) is an indispensable technique for the separation of high-molecular-weight analytes and for determining molar-mass distributions. The potential application of SEC as second-dimension separation in comprehensive two-dimensional liquid chromatography demands very short analysis times. Liquid chromatography benefits from the advent of highly efficient core-shell ...
متن کاملMethod Development for Size-Exclusion Chromatography of Monoclonal Antibodies and Higher Order Aggregates
in t ro du C t io n Since the early introduction of biologic based therapeutics, the presence of protein aggregates can compromise safety and efficacy.1 Given these factors, protein aggregates are typically monitored throughout the production of a biotherapeutic. While a variety of analytical techniques have been used to analyze soluble aggregates, the dominant technique continues to be size-ex...
متن کاملSlow-Equilibration Approximation in Kinetic Size Exclusion Chromatography.
Kinetic size exclusion chromatography with mass spectrometry detection (KSEC-MS) is a solution-based label-free approach for studying kinetics of reversible binding of a small molecule to a protein. Extraction of kinetic data from KSEC-MS chromatograms is greatly complicated by the lack of separation between the protein and protein-small molecule complex. As a result, a sophisticated time-consu...
متن کاملSize Exclusion Chromatography of Polymers
In the characterization of polymers, size-exclusion chromatography (SEC) has become a standard technique for determining molar mass averages andmolar mass distributions (MMD) of polymers. The principle of SEC is easily understood: owing to limited accessability of the pore volume within the particles of the column packing, polymer molecules are separated according to their hydrodynamic volumes,...
متن کاملRapid Miniaturized Chromatography Procedures for Iodinated Monoclonal Antibodies: Comparison to Gel Exclusion Chromatography
Chromatographic quality control testing of radioiodinated monoclonal antibodies (WJ MOAB) is necessary to assess radiochemical purity prior to patient injection. Conventional gel exclusion chromatography column scanning (GCS) is time consuming and not practical. We investigated rapid miniaturized chromatographic procedures for evaluating the radiochemical purity of 1311 MOAB. Three systems were...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2015
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2015.11.057